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Rearrangement of cytoskeletal elements, receptor expression and mitochondrial migration in human lens epithelial cells in culture

Priv.-Doz. Dr. rer. nat. Alfred Wegener
Heike Laser, MTA

 

Summary

Human capsulorhexis specimen from cataract lenses, obtained from surgery, are cultured in appropriate media on collagen coated glas slides for several weeks. Formation and rearrangement of ?-smooth muscle actin, vimentin and cytokeratin are studied with fluorescent antibody staining to investigate their role during formation of posterior capsular opacification. In addition expression of receptors for fibroblast growth factor (FGF) and endoglin are studied to evidence changes in susceptibility of the cells after leaving their capsular substrate.

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